Neighboring macrophage-induced alteration in the phenotype of colorectal cancer cells in the tumor budding area.

ABSTRACT:

Background

A higher number of tumor buds in the invasive front of colorectal cancer (CRC) specimens has been shown to contribute to a poor prognosis in CRC patients. Because macrophages (Mφs) have been demonstrated to alter the phenotype of cancer cells, we hypothesized that the phenotype of CRC cells in the tumor budding (TB) area might be changed by the interaction between CRC cells and Mφs.

Methods

We assessed the expression of topoisomerase 1 in CRC cells to estimate the acquisition of chemoresistance in CRC. To demonstrate the tumor-stromal interaction between CRC cells and Mφs, we assessed two histological findings, the number of Mφs per single CRC cell and the proximity between CRC cells and Mφs by histological spatial analysis using HALO software.

Results

The

SUBMITTER: Kawamura I 

PROVIDER: S-EPMC10938821 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature