A screen for regeneration-associated silencer regulatory elements in zebrafish.

ABSTRACT: Regeneration involves gene expression changes explained in part by context-dependent recruitment of transcriptional activators to distal enhancers. Silencers that engage repressive transcriptional complexes are less studied than enhancers and more technically challenging to validate, but they potentially have profound biological importance for regeneration. Here, we identified candidate silencers through a screening process that examined the ability of DNA sequences to limit injury-induced gene expression in larval zebrafish after fin amputation. A short sequence (s1) on chromosome 5 near several genes that reduce expression during adult fin regeneration could suppress promoter activity in stable transgenic lines and diminish nearby gene expression in knockin lines. High-resolution analysi

SUBMITTER: Ando K 

PROVIDER: S-EPMC10939760 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature