Ghrelin mediated cardioprotection using in vitro models of oxidative stress.

ABSTRACT: Ghrelin is commonly known as the 'hunger hormone' due to its role in stimulating food intake in humans. However, the roles of ghrelin extend beyond regulating hunger. Our aim was to investigate the ability of ghrelin to protect against hydrogen peroxide (H₂O₂), a reactive oxygen species commonly associated with cardiac injury. An in vitro model of oxidative stress was developed using H₂O₂ injured H9c2 cells. Despite lentiviral ghrelin overexpression, H9c2 cell viability and mitochondrial function were not protected following H₂O₂ injury. We found that H9c2 cells lack expression of the preproghrelin cleavage enzyme prohormone convertase 1 (encoded by PCSK1), required to convert ghrelin to its active form. In contrast, we foun

SUBMITTER: Kok CY 

PROVIDER: S-EPMC10940144 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature