RT-PCR assay to detect FGFR3::TACC3 fusions in formalin-fixed, paraffin-embedded glioblastoma samples.

ABSTRACT:

Background

One targeted treatment option for isocitrate dehydrogenase (IDH)-wild-type glioblastoma focuses on tumors with fibroblast growth factor receptor 3::transforming acidic coiled-coil-containing protein 3 (FGFR3::TACC3) fusions. FGFR3::TACC3 fusion detection can be challenging, as targeted RNA next-generation sequencing (NGS) is not routinely performed, and immunohistochemistry is an imperfect surrogate marker. Fusion status can be determined using reverse transcription polymerase chain reaction (RT-PCR) on fresh frozen (FF) material, but sometimes only formalin-fixed, paraffin-embedded (FFPE) tissue is available.

Aim

To develop an RT-PCR assay to determine FGFR3::TACC3 status in FFPE glioblastoma samples.

Methods

Twelve tissue micro

SUBMITTER: Priesterbach-Ackley LP 

PROVIDER: S-EPMC10940835 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature