Internalisation of neutrophils extracellular traps by macrophages aggravate rheumatoid arthritis via Rab5a.

ABSTRACT:

Objectives

Although elevated levels of neutrophil extracellular traps (NETs) have been reported in patients with rheumatoid arthritis (RA), the role of NETs in RA and the relationship between NETs and macrophages in the pathogenesis of RA requires further research. Here, we sought to determine the role of NETs in RA pathogenesis and reveal the potential mechanism.

Methods

Neutrophil elastase (NE) and myeloperoxidase (MPO)-DNA were measured in human serum and synovium. NETs inhibitor GSK484 was used to examine whether NETs involved with RA progression. We stimulated macrophages with NETs and detected internalisation-related proteins to investigate whether NETs entry into macrophages and induced inflammatory cytokines secretion through internalisation. To reveal mechanisms med

SUBMITTER: Ye H 

PROVIDER: S-EPMC10941157 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature