Characterization of cis-acting and NS1 protein-responsive elements in the p6 promoter of parvovirus B19.

ABSTRACT: Parvovirus B19 infections are associated with diverse clinical manifestations, ranging from no symptoms to severe symptoms. The virus shows an extreme tropism for replication in erythroid progenitor cells, possibly due to the activity of the only functional promoter (p6) of the B19 virus genome in combination with both cell- and cell cycle-specific factors and the trans-activator protein NS1. As presented here, p6 promoter sequences derived from several B19 virus isolates proved to be highly conserved. Furthermore, mutations did not affect any of the potential binding sites for transcription factors. One variation of the base at position 223 was identified only in B19 virus isolates derived from patients with persistent infection or chronic arthritis. To determine promoter activity and to

SUBMITTER: Gareus R 

PROVIDER: S-EPMC109414 | biostudies-literature | 1998 Jan

REPOSITORIES: biostudies-literature