Long-Term Porcine Retina Explants as an Alternative to In Vivo Experimentation.

ABSTRACT:

Purpose

The porcine retina represents an optimal model system to study treatment approaches for inherited retinal dystrophies owing to close anatomical similarities to the human retina, including a cone enriched visual streak. The aim of this work was to establish a protocol to keep explants in culture for up to 28 days with good morphological preservation.

Methods

Two to four retina explants per eye were obtained from the central part of the retina and transferred onto a membrane insert with the photoreceptors facing down. Different medium compositions using Neurobasal-A medium containing 100 or 450 mg/dL glucose and combinations of fetal calf serum, B-27 with or without insulin and N-2 were tested. We developed a tissue quality score with robust markers for different retin

SUBMITTER: Weller M 

PROVIDER: S-EPMC10941994 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature