A Human Brain Map of Mitochondrial Respiratory Capacity and Diversity.

ABSTRACT: Mitochondrial oxidative phosphorylation (OxPhos) powers brain activity^1,2, and mitochondrial defects are linked to neurodegenerative and neuropsychiatric disorders^3,4, underscoring the need to define the brain's molecular energetic landscape^5-10. To bridge the cognitive neuroscience and cell biology scale gap, we developed a physical voxelization approach to partition a frozen human coronal hemisphere section into 703 voxels comparable to neuroimaging resolution (3×3×3 mm). In each cortical and subcortical brain voxel, we profiled mitochondrial phenotypes including OxPhos enzyme activities, mitochondrial DNA and volume density, and mitochondria-specific respiratory capacity. We show that the human brain contains a diversity of mitochondrial phenotypes drive

SUBMITTER: Mosharov EV 

PROVIDER: S-EPMC10942385 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature