A transient brain endothelial translatome response to endotoxin is associated with mild cognitive changes post-shock in young mice.

ABSTRACT: Sepsis-associated encephalopathy (SAE) is a common manifestation in septic patients that is associated with increased risk of long-term cognitive impairment. SAE is driven, at least in part, by brain endothelial dysfunction in response to systemic cytokine signaling. However, the mechanisms driving SAE and its consequences remain largely unknown. Here, we performed translating ribosome affinity purification (TRAP) and RNA-sequencing (TRAP-seq) from the brain endothelium to determine the transcriptional changes after an acute endotoxemic (LPS) challenge. We found that LPS induces a strong acute transcriptional response in the brain endothelium that partially correlates with the whole brain transcriptional response and suggested an endothelial-specific hypoxia response. Consistent with a cri

SUBMITTER: Lu S 

PROVIDER: S-EPMC10942387 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature