Self-organization of embryonic stem cells into a reproducible embryo model through epigenome editing.

ABSTRACT: Embryonic stem cells (ESCs) can self-organize in vitro into developmental patterns with spatial organization and molecular similarity to that of early embryonic stages. This self-organization of ESCs requires transmission of signaling cues, via addition of small molecule chemicals or recombinant proteins, to induce distinct embryonic cellular fates and subsequent assembly into structures that can mimic aspects of early embryonic development. During natural embryonic development, different embryonic cell types co-develop together, where each cell type expresses specific fate-inducing transcription factors through activation of non-coding regulatory elements and interactions with neighboring cells. However, previous studies have not fully explored the possibility of engineering endoge

SUBMITTER: Lodewijk GA 

PROVIDER: S-EPMC10942404 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature