Protein complex heterogeneity and topology revealed by electron capture charge reduction and surface induced dissociation.

ABSTRACT: Herein, we focus on native mass spectrometry (nMS) combined with a fast, tunable gas-phase charge reduction, electron capture charge reduction (ECCR), and illustrate its utility in the characterization of protein complex topology and glycoprotein heterogeneity. ECCR is illustrated to effectively spread the charge states of tetradecameric GroEL, illustrating Orbitrap m/z measurement out to greater than 100,000 m/z. For both the pentameric C-reactive protein and tetradecameric GroEL, our novel device combining ECCR with surface induced dissociation (SID) lowers the charge states and produces more topologically informative fragmentation. While more native-like fragmentation has previously been illustrated for complexes charge reduced by proton abstraction in solution, this is th

SUBMITTER: Shaw J 

PROVIDER: S-EPMC10942452 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature