Nasopharyngeal metatranscriptomics reveals host-pathogen signatures of pediatric sinusitis.

ABSTRACT: Acute sinusitis (AS) is the fifth leading cause of antibiotic prescriptions in children. Distinguishing bacterial AS from common viral upper respiratory infections in children is crucial to prevent unnecessary antibiotic use but is challenging with current diagnostic methods. Despite its speed and cost, untargeted RNA sequencing of clinical samples from children with suspected AS has the potential to overcome several limitations of other methods. However, the utility of sequencing-based approaches in analysis of AS has not been fully explored. Here, we performed RNA-seq of nasopharyngeal samples from 221 children with clinically diagnosed AS to characterize their pathogen and host-response profiles. Results from RNA-seq were compared with those obtained using culture for three common bacte

SUBMITTER: AbuMazen N 

PROVIDER: S-EPMC10942525 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature