Rabies virus-based barcoded neuroanatomy resolved by single-cell RNA and in situ sequencing.

ABSTRACT: Mapping the connectivity of diverse neuronal types provides the foundation for understanding the structure and function of neural circuits. High-throughput and low-cost neuroanatomical techniques based on RNA barcode sequencing have the potential to map circuits at cellular resolution and a brain-wide scale, but existing Sindbis virus-based techniques can only map long-range projections using anterograde tracing approaches. Rabies virus can complement anterograde tracing approaches by enabling either retrograde labeling of projection neurons or monosynaptic tracing of direct inputs to genetically targeted postsynaptic neurons. However, barcoded rabies virus has so far been only used to map non-neuronal cellular interactions in vivo and synaptic connectivity of cultured neurons. Here we com

SUBMITTER: Zhang A 

PROVIDER: S-EPMC10942611 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature