A modified QuEChERS-based UPLC-MS/MS method for rapid determination of multiple antibiotics and sedative residues in freshwater fish.

ABSTRACT: Antibiotics and sedatives are used in freshwater fish culture and transportation, and residue in freshwater fish pose potential risks to human health. Therefore, a throughput method was developed to detect antibiotic and sedative residues in fish, simultaneously quantifying 68 antibiotics and 9 sedatives in freshwater fish using a modified QuEChERS extraction method and UPLC-MS/MS. Matrix-matched calibrations demonstrated good correlation coefficients (R² > 0.995), with a recovery range of 66.2-118.5%. The intra-day and inter-day relative standard deviation (RSD) were below 9.7% and 12.8%, respectively. The limits of detection (LOD) and quantification (LOQ) were 0.08-1.46 μg/kg and 0.25-4.86 μg/kg, respectively. 68.8% of analytes had weak matrix effects, and 13.0% had moderate m

SUBMITTER: Yang Y 

PROVIDER: S-EPMC10943039 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature