Proximity Mapping of Desmosomes Reveals a Striking Shift in Their Molecular Neighborhood Associated With Maturation.

ABSTRACT: Desmosomes are multiprotein adhesion complexes that link intermediate filaments to the plasma membrane, ensuring the mechanical integrity of cells across tissues, but how they participate in the wider signaling network to exert their full function is unclear. To investigate this, we carried out protein proximity mapping using biotinylation (BioID). The combined interactomes of the essential desmosomal proteins desmocollin 2a, plakoglobin, and plakophilin 2a (Pkp2a) in Madin-Darby canine kidney epithelial cells were mapped and their differences and commonalities characterized as desmosome matured from Ca²⁺ dependence to the mature, Ca²⁺-independent, hyper-adhesive state, which predominates in tissues. Results suggest that individual desmosomal proteins have distinct ro

SUBMITTER: Fulle JB 

PROVIDER: S-EPMC10943070 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature