CD4⁺CD25⁺ regulatory T cells ex vivo generated from autologous naive CD4⁺ T cells suppress EAE progression.

ABSTRACT: CD4⁺CD25⁺ regulatory T cells (Tregs) play an important role in maintaining immune homeostasis in multiple sclerosis (MS). Hence, we aimed to explore the therapeutic efficacy and safety of adoptive cell therapy (ACT) utilizing induced antigen-specific Tregs in an animal model of MS, that is, in an experimental autoimmune encephalomyelitis (EAE) model. B cells from EAE model that were activated with soluble CD40L were used as antigen-presenting cells (APCs) to induce the differentiation of antigen-specific Tregs from naïve CD4 precursors, and then, a stepwise isolation of CD4⁺CD25^highCD127^low Tregs was performed using a flow sorter. All EAE mice were divided into Treg-treated group (2 × 10⁴ cells in 0.2 mL per mouse, n = 14) a

SUBMITTER: Yang TT 

PROVIDER: S-EPMC10943184 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature