Pip5k1c expression in osteocytes regulates bone remodeling in mice.

ABSTRACT:

Research background

The role of osteocytes in maintaining bone mass has been progressively emphasized. Pip5k1c is the most critical isoform among PIP5KIs, which can regulate cytoskeleton, biomembrane, and Ca²⁺ release of cells and participate in many processes, such as cell adhesion, differentiation, and apoptosis. However, its expression and function in osteocytes are still unclear.

Materials and methods

To determine the function of Pip5k1c in osteocytes, the expression of Pip5k1c in osteocytes was deleted by breeding the 10-kb mouse Dmp1-Cre transgenic mice with the Pip5k1c^fl/fl mice. Bone histomorphometry, micro-computerized tomography analysis, immunofluorescence staining and western blotting were used to determine the effects o

SUBMITTER: Lin S 

PROVIDER: S-EPMC10943313 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature