Validation of the slaughterhouse porcine heart model for ex-situ heart perfusion studies.

ABSTRACT:

Introduction

To validate slaughterhouse hearts for ex-situ heart perfusion studies, we compared cold oxygenated machine perfusion in less expensive porcine slaughterhouse hearts (N = 7) to porcine hearts that are harvested following the golden standard in laboratory animals (N = 6).

Methods

All hearts received modified St Thomas 2 crystalloid cardioplegia prior to 4 hours of cold oxygenated machine perfusion. Hearts were perfused with homemade modified Steen heart solution with a perfusion pressure of 20-25 mmHg to achieve a coronary flow between 100-200 mL/min. Reperfusion and testing was performed for 4 hours on a normothermic, oxygenated diluted whole blood loaded heart model. Survival was defined by a cardiac output above 3 L with a mean aortic press

SUBMITTER: Kaffka Genaamd Dengler SE 

PROVIDER: S-EPMC10943619 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature