City-scale monitoring of antibiotic resistance genes by digital PCR and metagenomics.

ABSTRACT:

Background

Anthropogenic activities significantly contribute to the dissemination of antibiotic resistance genes (ARGs), posing a substantial threat to humankind. The development of methods that allow robust ARG surveillance is a long-standing challenge. Here, we use city-scale monitoring of ARGs by using two of the most promising cutting-edge technologies, digital PCR (dPCR) and metagenomics.

Methods

ARG hot-spots were sampled from the urban water and wastewater distribution systems. Metagenomics was used to provide a broad view of ARG relative abundance and richness in the prokaryotic and viral fractions. From the city-core ARGs in all samples, the worldwide dispersed sul2 and tetW conferring resistance to sulfonamide and tetracycline, respectively, were monitored by dPCR

SUBMITTER: Maestre-Carballa L 

PROVIDER: S-EPMC10943798 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature