Characterizing reference genes for high-fidelity gene expression analysis under different abiotic stresses and elicitor treatments in fenugreek leaves.

ABSTRACT:

Background

Quantifying gene expression is a critical aspect of applied genomics research across all organisms, and real-time PCR has emerged as a powerful tool for this purpose. However, selecting appropriate internal control genes for data normalization presents specific challenges. This study aimed to identify suitable reference genes for gene expression analysis under various conditions, encompassing salinity, low and high-temperature stresses, and different elicitor treatments. These treatments included titanium dioxide, cold plasma, 24-epibrassinolide, and melatonin, resulting in a total of 13 unique treatments and 148 treatment combinations applied to fenugreek plants.

Results

As per the analysis performed with the BestKeeper tool, EEF-1α, and GAPDH were recognized as

SUBMITTER: Ebrahimi A 

PROVIDER: S-EPMC10943880 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature