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Protocol to study circadian food-anticipatory poking in mice using the feeding experimentation device version 3.


ABSTRACT: Food-anticipatory nose poking is a unique food-seeking behavior driven by the food-entrainable oscillator. Here, we present a protocol to record a novel food-seeking nose poking behavior in mice under temporally restricted feeding followed by food deprivation using the open-source feeding experimentation device version 3 (FED3). We describe steps for setting up the FED3 and cage, training, and habituation. We then detail procedures for setting up the schedule for time-restricted feeding and food deprivation and for generating ethograms from FED3 data. For complete details on the use and execution of this protocol, please refer to Ehichioya et al.1.

SUBMITTER: Ehichioya DE 

PROVIDER: S-EPMC10943958 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature

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Protocol to study circadian food-anticipatory poking in mice using the feeding experimentation device version 3.

Ehichioya David E DE   Masud Ishrat I   Taufique S K Tahajjul SKT   Jeong Byeongha B   Farah Sofia S   Eischeid Averey A   Balaji Khaviya K   Shen Melody M   Takahashi Joseph S JS   Yamazaki Shin S  

STAR protocols 20240311 2


Food-anticipatory nose poking is a unique food-seeking behavior driven by the food-entrainable oscillator. Here, we present a protocol to record a novel food-seeking nose poking behavior in mice under temporally restricted feeding followed by food deprivation using the open-source feeding experimentation device version 3 (FED3). We describe steps for setting up the FED3 and cage, training, and habituation. We then detail procedures for setting up the schedule for time-restricted feeding and food  ...[more]

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