Dynamic establishment of recipient resident memory T cell repertoire after human intestinal transplantation.

ABSTRACT:

Background

Understanding formation of the human tissue resident memory T cell (TRM) repertoire requires longitudinal access to human non-lymphoid tissues.

Methods

By applying flow cytometry and next generation sequencing to serial blood, lymphoid tissue, and gut samples from 16 intestinal transplantation (ITx) patients, we assessed the origin, distribution, and specificity of human TRMs at phenotypic and clonal levels.

Findings

Donor age ≥1 year and blood T cell macrochimerism (peak level ≥4%) were associated with delayed establishment of stable recipient TRM repertoires in the transplanted ileum. T cell receptor (TCR) overlap between paired gut and blood repertoires from ITx patients was significantly greater than that in healthy controls, demonstrating increased gut

SUBMITTER: Jiao W 

PROVIDER: S-EPMC10944178 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature