Modular air-liquid interface aerosol exposure system (MALIES) to study toxicity of nanoparticle aerosols in 3D-cultured A549 cells in vitro.

ABSTRACT: We present a novel lung aerosol exposure system named MALIES (modular air-liquid interface exposure system), which allows three-dimensional cultivation of lung epithelial cells in alveolar-like scaffolds (MatriGrids^®) and exposure to nanoparticle aerosols. MALIES consists of multiple modular units for aerosol generation, and can be rapidly assembled and commissioned. The MALIES system was proven for its ability to reliably produce a dose-dependent toxicity in A549 cells using CuSO₄ aerosol. Cytotoxic effects of BaSO₄- and TiO₂-nanoparticles were investigated using MALIES with the human lung tumor cell line A549 cultured at the air-liquid interface. Experiments with concentrations of up to 5.93 × 10⁵ (BaSO₄) and 1.49 × 10

SUBMITTER: Kustner MJ 

PROVIDER: S-EPMC10944414 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature