Distinct concentration-dependent oxidative stress profiles by cadmium in a rat kidney proximal tubule cell line.

ABSTRACT: Levels and chemical species of reactive oxygen/nitrogen species (ROS/RNS) determine oxidative eustress and distress. Abundance of uptake pathways and high oxygen consumption for ATP-dependent transport makes the renal proximal tubule particularly susceptible to cadmium (Cd²⁺)-induced oxidative stress by targeting ROS/RNS generation or antioxidant defence mechanisms, such as superoxide dismutase (SOD) or H₂O₂-metabolizing catalase (CAT). Though ROS/RNS are well-evidenced, the role of distinct ROS profiles in Cd²⁺ concentration-dependent toxicity is not clear. In renal cells, Cd²⁺ (10-50 µM) oxidized dihydrorhodamine 123, reaching a maximum at 2-3 h. Increases (up to fourfold) in lipid peroxidation by TBARS assay and H₂O₂

SUBMITTER: Lee WK 

PROVIDER: S-EPMC10944451 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature