Assessment of cellular senescence potential of PM2.5 using 3D human lung fibroblast spheroids in vitro model.

ABSTRACT:

Background

Epidemiological studies demonstrate that particulate matter 2.5 (PM2.5) exposure closely related to chronic respiratory diseases. Cellular senescence plays an important role in many diseases. However, it is not fully clear whether PM2.5 exposure could induce cellular senescence in the human lung. In this study, we generated a three-dimensional (3D) spheroid model using isolated primary human lung fibroblasts (HLFs) to investigate the effects of PM2.5 on cellular senescence at the 3D level.

Methods

3D spheroids were exposed to 25-100 μg/ml of PM2.5 in order to evaluate the impact on cellular senescence. SA-β-galactosidase activity, cell proliferation, and the expression of key genes and proteins were detected.

Results

Exposure of the HLF spheroids to PM2.5 y

SUBMITTER: Xu S 

PROVIDER: S-EPMC10944558 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature