A single workflow for multi-species blood transcriptomics.

ABSTRACT:

Background

Blood transcriptomic analysis is widely used to provide a detailed picture of a physiological state with potential outcomes for applications in diagnostics and monitoring of the immune response to vaccines. However, multi-species transcriptomic analysis is still a challenge from a technological point of view and a standardized workflow is urgently needed to allow interspecies comparisons.

Results

Here, we propose a single and complete total RNA-Seq workflow to generate reliable transcriptomic data from blood samples from humans and from animals typically used in preclinical models. Blood samples from a maximum of six individuals and four different species (rabbit, non-human primate, mouse and human) were extracted and sequenced in triplicates. The workflow was eva

SUBMITTER: Orcel E 

PROVIDER: S-EPMC10944614 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature