Optimal selection of specimens for metagenomic next-generation sequencing in diagnosing periprosthetic joint infections.

ABSTRACT:

Objective

This study aimed to assess the diagnostic value of metagenomic next-generation sequencing (mNGS) across synovial fluid, prosthetic sonicate fluid, and periprosthetic tissues among patients with periprosthetic joint infection (PJI), intending to optimize specimen selection for mNGS in these patients.

Methods

This prospective study involved 61 patients undergoing revision arthroplasty between September 2021 and September 2022 at the First Affiliated Hospital of Zhengzhou University. Among them, 43 cases were diagnosed as PJI, and 18 as aseptic loosening (AL) based on the American Musculoskeletal Infection Society (MSIS) criteria. Preoperative or intraoperative synovial fluid, periprosthetic tissues, and prosthetic sonicate fluid were collected, each divided into two

SUBMITTER: Tan J 

PROVIDER: S-EPMC10945027 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature