Comparison of three-dimensional cell culture techniques of dedifferentiated liposarcoma and their integration with future research.

ABSTRACT: Background: Dedifferentiated liposarcoma is a formidable sarcoma subtype due to its high local recurrence rate and resistance to medical treatment. While 2D cell cultures are still commonly used, 3D cell culture systems have emerged as a promising alternative, particularly scaffold-based techniques that enable the creation of 3D models with more accurate cell-stroma interactions. Objective: To investigate how 3D structures with or without the scaffold existence would affect liposarcoma cell lines growth morphologically and biologically. Methods: Lipo246 and Lipo863 cell lines were cultured in 3D using four different methods; Matrigel^® ECM scaffold method, Collagen ECM scaffold method, ULA plate method and Hanging drop method, in addition to conventional 2D c

SUBMITTER: Tahara S 

PROVIDER: S-EPMC10945377 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature