Intrinsic protein disorder is insufficient to drive subnuclear clustering in embryonic transcription factors.

ABSTRACT: Modern microscopy has revealed that core nuclear functions, including transcription, replication, and heterochromatin formation, occur in spatially restricted clusters. Previous work from our lab has shown that subnuclear high-concentration clusters of transcription factors may play a role in regulating RNA synthesis in the early Drosophila embryo. A nearly ubiquitous feature of eukaryotic transcription factors is that they contain intrinsically disordered regions (IDRs) that often arise from low complexity amino acid sequences within the protein. It has been proposed that IDRs within transcription factors drive co-localization of transcriptional machinery and target genes into high-concentration clusters within nuclei. Here, we test that hypothesis directly, by conducting a broad s

SUBMITTER: Hannon CE 

PROVIDER: S-EPMC10945700 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature