TRNA epitranscriptome determines pathogenicity of the opportunistic pathogen Pseudomonas aeruginosa.

ABSTRACT: The success of bacterial pathogens depends on the coordinated expression of virulence determinants. Regulatory circuits that drive pathogenesis are complex, multilayered, and incompletely understood. Here, we reveal that alterations in tRNA modifications define pathogenic phenotypes in the opportunistic pathogen Pseudomonas aeruginosa. We demonstrate that the enzymatic activity of GidA leads to the introduction of a carboxymethylaminomethyl modification in selected tRNAs. Modifications at the wobble uridine base (cmnm⁵U₃₄) of the anticodon drives translation of transcripts containing rare codons. Specifically, in P. aeruginosa the presence of GidA-dependent tRNA modifications modulates expression of genes encoding virulence regulators, leading to a cellu

SUBMITTER: Krueger J 

PROVIDER: S-EPMC10945773 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature