Exploiting spatiotemporal regulation of FZD5 during neural patterning for efficient ventral midbrain specification.

ABSTRACT: The Wnt/β-catenin signaling governs anterior-posterior neural patterning during development. Current human pluripotent stem cell (hPSC) differentiation protocols use a GSK3 inhibitor to activate Wnt signaling to promote posterior neural fate specification. However, GSK3 is a pleiotropic kinase involved in multiple signaling pathways and, as GSK3 inhibition occurs downstream in the signaling cascade, it bypasses potential opportunities for achieving specificity or regulation at the receptor level. Additionally, the specific roles of individual FZD receptors in anterior-posterior patterning are poorly understood. Here, we have characterized the cell surface expression of FZD receptors in neural progenitor cells with different regional identity. Our data reveal unique upregulation of FZD5 exp

SUBMITTER: Yang A 

PROVIDER: S-EPMC10946437 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature