Cysteine-Selective Modification of Peptides and Proteins via Desulfurative C-C Bond Formation.

ABSTRACT: The site-selective modification of peptides and proteins facilitates the preparation of targeted therapeutic agents and tools to interrogate biochemical pathways. Among the numerous bioconjugation techniques developed to install groups of interest, those that generate C(sp³ )-C(sp³ ) bonds are significantly underrepresented despite affording proteolytically stable, biogenic linkages. Herein, a visible-light-mediated reaction is described that enables the site-selective modification of peptides and proteins via desulfurative C(sp³ )-C(sp³ ) bond formation. The reaction is rapid and high yielding in peptide systems, with comparable translation to proteins. Using this chemistry, a range of moieties is installed into model systems and an effective PT

SUBMITTER: Griffiths RC 

PROVIDER: S-EPMC10946470 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature