Progenitor cell-derived basophils: A novel barcoded passive degranulation assay in allergic diseases.

ABSTRACT:

Background

Effector cells assays provide an overall measure of responsiveness to allergen, but the lack of reliable and high-throughput assays limits the clinical utility. We aimed to develop a high-throughput basophil activation test based on human progenitor cell-derived basophils (PCB) and investigate the role of PCB activation test (PCBAT) in allergic diseases.

Methods

Progenitor cell-derived basophils were differentiated from CD34⁺ progenitor cells and sensitized with sera from subjects sensitized to cat, peanut or atopic controls. Sensitized PCBs were stimulated with increasing concentrations of the corresponding allergens in vitro. Degranulation was assessed by measuring CD63 expression using flow cytometry. The correlations between PCBAT and clinical aller

SUBMITTER: Wu J 

PROVIDER: S-EPMC10946533 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature