Engineering an Oxygen-Binding Protein for Photocatalytic CO₂ Reductions in Water.

ABSTRACT: While native CO₂ -reducing enzymes display remarkable catalytic efficiency and product selectivity, few artificial biocatalysts have been engineered to allow understanding how the native enzymes work. To address this issue, we report cobalt porphyrin substituted myoglobin (CoMb) as a homogeneous catalyst for photo-driven CO₂ to CO conversion in water. The activity and product selectivity were optimized by varying pH and concentrations of the enzyme and the photosensitizer. Up to 2000 TON(CO) was attained at low enzyme concentrations with low product selectivity (15 %), while a product selectivity of 74 % was reached by increasing the enzyme loading but with a compromised TON(CO). The efficiency of CO generation and overall TON(CO) were further improved by introducing

SUBMITTER: Deng Y 

PROVIDER: S-EPMC10946749 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature