Probing Peptidylprolyl Bond cis/trans Status Using Distal ¹⁹ F NMR Reporters.

ABSTRACT: A method for measuring peptidylprolyl bond cis-trans conformational status in peptide models is described, using 4-fluorophenylalanine (4FPhe) as a distal reporter for ¹⁹ F NMR. The %cis-Pro population was measured for peptides of the general structure Ac-X-Pro-Z-Ala-Ala-4FPhe (X and Z are proteinogenic amino acids) at pH 7.4, and provided conformational populations consistent with literature values obtained by more complex methods. This approach was applied to probe the prolyl bond status in pentapeptide models of the intrinsically disordered C-terminal region of α-synuclein, which mirrored the preferences in the Ac-X-Pro-Z-Ala-4FPhe models. Advantageously, the ¹⁹ F reporter group does not need to be adjacent to or attached to proline to provide quantifiable signals

SUBMITTER: Killoran PM 

PROVIDER: S-EPMC10946801 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature