Characterization of acrosin and acrosin binding protein as novel CRISP2 interacting proteins in boar spermatozoa.

ABSTRACT:

Background

Previously, we reported that cysteine-rich secretory protein 2 is involved in high molecular weight complexes in boar spermatozoa. These cysteine-rich secretory protein 2protein complexes are formed at the last phase of sperm formation in the testis and play a role in sperm shaping and functioning.

Objectives

This study aimed to identify cysteine-rich secretory protein 2 interacting partners. These binding partner interactions were investigated under different conditions, namely, non-capacitating conditions, after the induction of in vitro sperm capacitation and subsequently during an ionophore A23187-induced acrosome reaction.

Materials and methods

The incubated pig sperm samples were subjected to protein extraction. Extracted proteins were subjected to bl

SUBMITTER: Zhang M 

PROVIDER: S-EPMC10947329 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature