Control of human pancreatic beta cell kinome by glucagon-like peptide-1 receptor biased agonism.

ABSTRACT:

Aim

To determine the kinase activity profiles of human pancreatic beta cells downstream of glucagon-like peptide-1 receptor (GLP-1R) balanced versus biased agonist stimulations.

Materials and methods

This study analysed the kinomic profiles of human EndoC-βh1 cells following vehicle and GLP-1R stimulation with the pharmacological agonist exendin-4, as well as exendin-4-based biased derivatives exendin-phe1 and exendin-asp3 for acute (10-minute) versus sustained (120-minute) responses, using PamChip protein tyrosine kinase and serine/threonine kinase assays. The raw data were filtered and normalized using BioNavigator. The kinase analyses were conducted with R, mainly including kinase-substrate mapping and Kyoto Encyclopedia of Genes and Genomes pathway analysis.

Results</

SUBMITTER: Xiao J 

PROVIDER: S-EPMC10947446 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature