Single Cell Forces after Electroporation.

ABSTRACT: Exogenous high-voltage pulses increase cell membrane permeability through a phenomenon known as electroporation. This process may also disrupt the cell cytoskeleton causing changes in cell contractility; however, the contractile signature of cell force after electroporation remains unknown. Here, single-cell forces post-electroporation are measured using suspended extracellular matrix-mimicking nanofibers that act as force sensors. Ten, 100 μs pulses are delivered at three voltage magnitudes (500, 1000, and 1500 V) and two directions (parallel and perpendicular to cell orientation), exposing glioblastoma cells to electric fields between 441 V cm^-1 and 1366 V cm^-1. Cytoskeletal-driven force loss and recovery post-electroporation involves three distinct stages. Low elec

SUBMITTER: Graybill PM 

PROVIDER: S-EPMC10949415 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature