Activation of Piezo1 channels in compressed red blood cells augments platelet-driven contraction of blood clots.

ABSTRACT:

Background

Piezo1 is a mechanosensitive cationic channel that boosts intracellular [Ca²⁺]_i. Compression of red blood cells (RBCs) during platelet-driven contraction of blood clots may cause the activation of Piezo1.

Objectives

To establish relationships between Piezo1 activity and blood clot contraction.

Methods

Effects of a Piezo1 agonist, Yoda1, and antagonist, GsMTx-4, on clot contraction in vitro were studied in human blood containing physiological [Ca²⁺]. Clot contraction was induced by exogenous thrombin. Activation of Piezo1 was assessed by Ca²⁺ influx in RBCs and with other functional and morphologic features.

Results

Piezo1 channels in compressed RBCs are activated naturally during blood clot contraction and

SUBMITTER: Evtugina NG 

PROVIDER: S-EPMC10949619 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature