Anaplastic thyroid cancer spheroids as preclinical models to test therapeutics.

ABSTRACT: Anaplastic thyroid cancer (ATC) is the most aggressive thyroid cancer. Despite advances in tissue culture techniques, a robust model for ATC spheroid culture is yet to be developed. In this study, we created an efficient and cost-effective 3D tumor spheroids culture system from human ATC cells and existing cell lines that better mimic patient tumors and that can enhance our understanding of in vivo treatment response. We found that patient-derived ATC cells and cell lines can readily form spheroids in culture with a unique morphology, size, and cytoskeletal organization. We observed both cohesive (dense and solid structures) and discohesive (irregularly shaped structures) spheroids within the same culture condition across different cell lines. BRAF^WT ATC spheroids grew in a cohe

SUBMITTER: Hu J 

PROVIDER: S-EPMC10949686 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature