MiR-430 regulates zygotic mRNA during zebrafish embryogenesis.

ABSTRACT:

Background

Early embryonic developmental programs are guided by the coordinated interplay between maternally inherited and zygotically manufactured RNAs and proteins. Although these processes happen concomitantly and affecting gene function during this period is bound to affect both pools of mRNAs, it has been challenging to study their expression dynamics separately.

Results

By employing SLAM-seq, a nascent mRNA labeling transcriptomic approach, in a developmental time series we observe that over half of the early zebrafish embryo transcriptome consists of maternal-zygotic genes, emphasizing their pivotal role in early embryogenesis. We provide an hourly resolution of de novo transcriptional activation events and follow nascent mRNA trajectories, finding that most de novo t

SUBMITTER: Baia Amaral D 

PROVIDER: S-EPMC10949700 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature