Engineered extracellular vesicles carrying let-7a-5p for alleviating inflammation in acute lung injury.

ABSTRACT:

Background

Acute lung injury (ALI) is a life-threatening respiratory condition characterized by severe inflammation and lung tissue damage, frequently causing rapid respiratory failure and long-term complications. The microRNA let-7a-5p is involved in the progression of lung injury, inflammation, and fibrosis by regulating immune cell activation and cytokine production. This study aims to use an innovative cellular electroporation platform to generate extracellular vesicles (EVs) carring let-7a-5p (EV-let-7a-5p) derived from transfected Wharton's jelly-mesenchymal stem cells (WJ-MSCs) as a potential gene therapy for ALI.

Methods

A cellular nanoporation (CNP) method was used to induce the production and release of EV-let-7a-5p from WJ-MSCs transfected with the relevant plasmi

SUBMITTER: Chen SY 

PROVIDER: S-EPMC10949767 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature