Distinct molecular profiles drive multifaceted characteristics of colorectal cancer metastatic seeds.

ABSTRACT: Metastasis of primary tumors remains a challenge for early diagnosis and prevention. The cellular properties and molecular drivers of metastatically competent clones within primary tumors remain unclear. Here, we generated 10-16 single cell-derived lines from each of three colorectal cancer (CRC) tumors to identify and characterize metastatic seeds. We found that intrinsic factors conferred clones with distinct metastatic potential and cellular communication capabilities, determining organ-specific metastasis. Poorly differentiated or highly metastatic clones, rather than drug-resistant clones, exhibited poor clinical prognostic impact. Personalized genetic alterations, instead of mutation burden, determined the occurrence of metastatic potential during clonal evolution. Additionally, we d

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC10949939 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature