Goat milk extracellular vesicles: Separation comparison of natural carriers for theragnostic application.

ABSTRACT: Goat milk is a complex biological fluid, which in addition to having a high nutritional value, it is an interesting source of extracellular vesicles (EVs). Despite the countless potential applications that they offer in many biological fields, is not easy to compare the different proposed systems, and this is a major limitation for the real translatability of these natural nanoplatforms for theragnostic purposes. Thus, it is useful to further investigate reproducible methods to separate goat milk EVs. The choice of methods but also the preprocessing of milk has an immense impact on the separation, quality, and yield of EVs. Here, we tested four protocols to separate EVs from unpasteurised goat milk: two based on differential ultracentrifugation (DUC) and two on size-exclusion chromatograph

SUBMITTER: Santoro J 

PROVIDER: S-EPMC10950560 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature