Moving towards on-site detection of Shiga toxin-producing Escherichia coli in ready-to-eat leafy greens.

ABSTRACT: Rapid identification of Shiga toxin-producing Escherichia coli, or STEC, is of utmost importance to assure the innocuousness of the foodstuffs. STEC have been implicated in outbreaks associated with different types of foods however, among them, ready-to-eat (RTE) vegetables are particularly problematic as they are consumed raw, and are rich in compounds that inhibit DNA-based detection methods such as qPCR. In the present study a novel method based on Loop-mediated isothermal amplification (LAMP) to overcome the limitations associated with current molecular methods for the detection of STEC in RTE vegetables targeting stx1 and stx2 genes. In this sense, LAMP demonstrated to be more robust against inhibitory substances in food. In this study, a comprehensive enrichment

SUBMITTER: Costa-Ribeiro A 

PROVIDER: S-EPMC10950744 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature