Short turnaround time of seven to nine hours from sample collection until informed decision for sepsis treatment using nanopore sequencing.

ABSTRACT: Bloodstream infections (BSIs) and sepsis are major health problems, annually claiming millions of lives. Traditional blood culture techniques, employed to identify sepsis-causing pathogens and assess antibiotic susceptibility, usually take 2-4 days. Early and accurate antibiotic prescription is vital in sepsis to mitigate mortality and antibiotic resistance. This study aimed to reduce the wait time for sepsis diagnosis by employing shorter blood culture incubation times for BD BACTEC™ bottles using standard laboratory incubators, followed by real-time nanopore sequencing and data analysis. The method was tested on nine blood samples spiked with clinical isolates from the six most prevalent sepsis-causing pathogens. The results showed that pathogen identification was possible at as low as 1

SUBMITTER: Ali J 

PROVIDER: S-EPMC10951244 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature