Optical Estimation of Membrane Potential Values Using Fluorescence Lifetime Imaging Microscopy and Hybrid Chemical-Genetic Voltage Indicators.

ABSTRACT:

Introduction

Membrane potential (V_m), the voltage across a cell membrane, is an important biophysical phenomenon, central to the physiology of cells, tissues, and organisms. Voltage-sensitive fluorescent indicators are a powerful method for interrogating membrane potential in living systems, but most indicators are best suited for detecting changes in membrane potential rather than measuring values of the membrane potential. One promising approach is to use fluorescence lifetime imaging microscopy (FLIM) in combination of chemically synthesized dyes to estimate a value of membrane potential. However, a drawback is that chemically synthesized dyes show poor specificity of staining.

Objectives

To address this problem, we applied a chemical-genetic voltage ima

SUBMITTER: Gest AMM 

PROVIDER: S-EPMC10951690 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature