Scipion3: A workflow engine for cryo-electron microscopy image processing and structural biology.

ABSTRACT: Image-processing pipelines require the design of complex workflows combining many different steps that bring the raw acquired data to a final result with biological meaning. In the image-processing domain of cryo-electron microscopy single-particle analysis (cryo-EM SPA), hundreds of steps must be performed to obtain the three-dimensional structure of a biological macromolecule by integrating data spread over thousands of micrographs containing millions of copies of allegedly the same macromolecule. The execution of such complicated workflows demands a specific tool to keep track of all these steps performed. Additionally, due to the extremely low signal-to-noise ratio (SNR), the estimation of any image parameter is heavily affected by noise resulting in a significant fraction of incorrect

SUBMITTER: Conesa P 

PROVIDER: S-EPMC10951921 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature