Modification of the swirling well cell culture model to alter shear stress metrics.

ABSTRACT: Effects of hemodynamic shear stress on endothelial cells have been extensively investigated using the "swirling well" method, in which cells are cultured in dishes or multiwell plates placed on an orbital shaker. A wave rotates around the well, producing complex patterns of shear. The method allows chronic exposure to flow with high throughput at low cost but has two disadvantages: a number of shear stress characteristics change in a broadly similar way from the center to the edge of the well, and cells at one location in the well may release mediators into the medium that affect the behavior of cells at other locations, exposed to different shears. These properties make it challenging to correlate cell properties with shear. The present study investigated simple alterations to ameliorate

SUBMITTER: Arshad M 

PROVIDER: S-EPMC10952219 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature