MORC3 represses the HCMV major immediate early promoter in myeloid cells in the absence of PML nuclear bodies.

ABSTRACT: Human cytomegalovirus (HCMV) can undergo either a latent or a lytic infection in cells of the myeloid lineage. Whilst the molecular mechanisms which determine the outcome of infection are far from clear, it is well established that a key factor is the differential regulation of the major immediate early promoter (MIEP) responsible for driving lytic immediate early gene expression. Using a myelomonocytic cell line stably transduced with a GFP reporter under the control of the MIEP, which recapitulates MIEP regulation in the context of virus infection, we have used an unbiased CRISPR-Cas9 sub-genomic, epigenetic library screen to identify novel cellular factors involved in MIEP repression during establishment and maintenance of latency in myeloid cells. One such cellular factor identified wa

SUBMITTER: Champion A 

PROVIDER: S-EPMC10952291 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature